2009 Undergraduate Researchers
- Bryce Bushe
Brodl Research Group
Biological responses to environmental stress profoundly impact normal developmental processes such as pattern formation, cell-cell interaction, and processes that depend on secretion. My laboratory investigates the heat stress response of barley and rice aleurone cells, focusing on the cellular and molecular mechanisms these secretory cells use to redirect their normal cellular activities toward coping with heat. Heat shock causes a collapse and rebuilding of the ER (see Figure), and the mRNA that encodes the secretory proteins that are normally ER-bound during their translation are selectively destabilized (nonsecretory protein mRNAs remain stable). We isolate ER by ultracentrifugation on sucrose gradients and analyze its protein and lipid composition, which we can also manipulate biochemically. We use translation in vitro to monitor the ER’s ability to import proteins under stress conditions. We investigate the mechanism for targeted secretory protein mRNA decay by genetically engineering genes to either include or remove sequences that are candidates for regulating mRNA stability. Microprojectile bombardment is then used to genetically transform aleurone cells with these constructs, and we monitor mRNA levels in transformants by real-time PCR and/or standard RT-PCR. This has led us to the identification of an RNA binding protein that may be important in targeted decay. We are isolating this protein to use reverse genetics approaches to identify it for later transgenic studies. In the end, the insights gained from understanding the mechanisms underlying stress responses will help guide breeding and genetic engineering efforts to improve sustainability.
Qualifications of successful applicants
Students should have completed at least two semesters of both biology and chemistry. Strong GPAs are not as important as a solid work ethic matched with strong dedication and interest. At the same time, GPAs are broadly indicative of these traits. Students work independently on their projects in my lab, so excellent planning, meticulous note-taking and keen attention to detail are critical attributes. Ideally, in the spring semester students will undertake an independent study (about 1 semester hour) to begin reading background literature and to begin learning fundamental experimental techniques. Previous research experience is not required.
