Laboratory Schedule for November 13,
2007
NOTE: Try to be in lab (CLS #135) ~10
minutes before your scheduled time.
NOTE: The Centrifuge is in
CLS #156. Get your tubes in and start as
fast as possible.
NOTE: It will be a long day if you visit too much during this lab exercise.
HOMOGENIZATION TIMES
TIME STUDENT OVARY
NUMBER OTHER DUTIES
01:00 Espey Tubes: 4-1, 4-2 -----
01:05
01:15 Kennedy Tubes: 0-1, 0-2, 0-3 Teach the next group
01:20 CENTRIFUGE (at 5,000 rpm for 10 minutes)
01:25
01:30 Furr Tubes:
24-3, 24-4 Centrifuge
01:35 Hampton Tubes: 24-5, 24-6 Teach the next group
01:40 CENTRIFUGE (at 5,000 rpm for 10 minutes)
01:45 Anhalt Tubes: I-1, I-2, I-3 Centrifuge
01:55 Goebel Tubes: I-4, I-5, I-6 Teach the next group
02:00 CENTRIFUGE (at 5,000 rpm for 10 minutes)
02:00 Espey Tubes: 4-3 -----
02:05
02:10 Dingwall Tubes: 12-1, 12-2, 12-3 Teach the next group
02:20 CENTRIFUGE (at 5,000 rpm for 10 minutes)
02:25 Comer Tubes: 12-4,12-5, 12-6 Centrifuge
02:35 Edwards Tubes: 0-4, 0-5, 0-6 Teach the next group
02:40 CENTRIFUGE (at 5,000 rpm for 10 minutes)
02:45 McCarthy Tubes: 8-3, 8-4 Centrifuge
02:50 Morris Tubes: 8-5, 8-6 -----
03:00 CENTRIFUGE (at 5,000 rpm for 10 minutes)
NOTE: After
centrifugation, pipet 500 ml of supernatant fluid from your homogenate into a fresh
tube that has been labeled for you.
Then, add 2.5 ml of
distilled water to this 500 ml of supernatant. Next, follow
the radioimmunoassay procedure outlined in "Exercise
#1" in the website instructions for this course.
NOTE: During the 40-minute incubation at
37°C of your progesterone-assay tubes, you will perform the first steps
of the prostaglandin RIA and then use the spectrophotometer to determine the
absorbance (i.e., optical density) of your ovarian extract.