IL-10


 

Description

IL-10 is a cytokine expressed mainly by monocytes with multiple, pleiotropic effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages; it also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4+ T cell activation, but further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) and bacterial product mediated induction of the pro-inflammatory cytokines TNF-α, IL-1β, IL-12, and IFN-g secretion from Toll-Like Receptor (TLR) triggered myeloid lineage cells. IL-10 and related cytokines (IL-22) exert essential functions to maintain tissue homeostasis during infection and inflammation through restriction of excessive inflammatory responses, upregulation of innate immunity, and promotion of tissue repairing mechanisms. Their important functions in diseases are supported by data from many preclinical models, human genetic studies, and clinical interventions (Ouyang and O'Garra 2019). There are no available reagents that identify marmoset IL-10.

 

Alignment

Protein alignment for human, rhesus macaque and marmoset IL-10:

image of Protein alignment for human, rhesus macaque and marmoset IL-10

 

Protein alignment for marmoset, owl monkey, and squirrel monkey IL-10:

Protein alignment for marmoset, owl monkey, and squirrel monkey IL-10

 

References

  • Ouyang, W. and A. O'Garra (2019). "IL-10 Family Cytokines IL-10 and IL-22: from Basic Science to Clinical Translation." Immunity 50(4): 871-891.

 

Status

Pure MT-3515 (capture) and biotinylated MT-32AD (detection) have been validated in ELISA, ELISPOT, and Luminex formats. Large scale antibody production is in progress.