We are fascinated by the ways in which molecules stick together.
In living systems, there are many thousands of different types of molecules, each with its own shape and polarity, and yet each molecule knows where to go—that is, it "recognizes" its partner and sticks to it selectively; this molecular recognition is a central function of biochemistry. We study the recognition of proteins by synthetic organic compounds in order to help us better understand protein interactions in living systems and to develop molecules that can control these processes. This is the foundation for medicinal chemistry and clinical diagnostics. Students are involved in every aspect of the projects, including designing and implementing the experiments, analyzing the data, and communicating the work through publications and presentations.
Students in this research group become proficient in a variety of techniques, even in their first year of college. These can include: 1) organic synthesis to make peptides and other small molecules; 2) protein expression and purification using molecular biology techniques; 3) protein semisynthesis; 3) NMR spectroscopy, mass spectrometry, and X-ray crystallography to characterize molecular structure; 3) microcalorimetry, stopped-flow spectroscopy, and gel electrophoresis to measure the thermodynamics and kinetics of binding; 4) UV-visible, fluorescence, and circular dichroism spectroscopy to study electronic and structural properties; and 5) computational modeling of intermolecular interactions. This combination of methods paints a detailed picture of the chemical processes we study, and it offers students a breadth of quantitative and qualitative technical skills and depth of insight that is an excellent foundation for further work in the areas of organic chemistry, biophysical chemistry, medicinal chemistry, biochemistry, bioengineering, and biotechnology.
An introduction for new students is available at the link at the bottom of this page. Please contact Dr. Urbach to learn more about our research and to inquire about joining the group.
We work primarily in the field of supramolecular chemistry, which is concerned with the interactions between molecules. These intermolecular interactions are driven by physical forces such as ionic interactions, dipole-dipole interactions, van der Waals forces, and the hydrophobic effect. Intermolecular interactions are fundamental to biochemistry and are required to form functional biological structures, such as proteins and nucleic acids, ribosomes, viral capsids, cell membranes, and organelles. Most processes in biology also require the cooperation between molecules, which involves their selective, noncovalent interaction. The molecular partners “recognize” one another because they have complementary shapes and polarities, allowing them to fit together and stick tightly.
Protein * DNA Complex
Supramolecular chemists have long sought to mimic the molecular recognition that occurs in biological systems in order to develop principles by which we can design synthetic molecules to recognize biological molecules and to construct new, functional entities of matter. Drugs are an excellent example; they are typically small organic molecules that bind to proteins in order to block their natural functions. The Urbach group is interested in organic molecules that bind to proteins in a predictable way. A protein is a chain of amino acids, and there are 20 different amino acids. The genetic code dictates the sequence of amino acids, and therefore this information is easy to obtain. The sequence of amino acids determines how the protein will fold into its functional form, but it remains very difficult to predict how a given sequence of amino acids will fold.
Drug * Protein Complex
The Urbach group seeks to develop an understanding of how to recognize a protein using only the information available in its amino acid sequence. We use “synthetic receptors”, which are relatively small molecules that have their own internal cavities in which other molecules can fit. Synthetic receptors work like antibodies but are less expensive, more stable, and easier to study. Few synthetic receptors, however, can bind with the strength and selectivity of antibodies, but we have discovered that the cucurbit[n]urils can do this. Cucurbit[n]urils are donut-shaped molecules that have a hydrophobic inner cavity with polar entrances on either side. Cucurbit[n]urils bind many organic cations by including the nonpolar portion of the molecule within the hydrophobic cavity, while the cationic groups interact with the polar entrances. Cucurbit[n]urils have gained a lot of attention in recent years due to their ability to function in water, their low toxicity and high stability, and their ability to bind to wide range of interesting molecules.
We discovered that cucurbit[n]urils can bind to the aromatic amino acids—tryptophan, phenylalanine, and tyrosine—when they are located at the first position in the protein chain. The nonpolar aromatic group binds inside the cucurbit[n]uril cavity, and the positive charge at the tail end of the protein (the nitrogen-terminus) binds to the polar entrance. These structural features allow for cucurbit[n]urils to bind predictably to proteins based on the identity of the first amino acid in the chain. Our work continues to expand on this discovery by pushing the boundaries of affinity and selectivity in the sequence-based molecular recognition of proteins and developing tools for biochemistry and biotechnology.
Crystal structure of the complex between
cucurbituril (grey) and insulin (green)